T-01
TB-500 reconstitution calculator
Concentration, per-dose volume, U-100 syringe units and doses per vial, from vial size, diluent volume and dose mass.
TB-500 has no label, so there are no official mixing constants to preload: every number here is arithmetic on values you enter, not product guidance. The calculator turns a stated vial strength and a diluent volume into a concentration, then turns any dose you document into an injection volume and a mark on a U-100 insulin syringe. It exists because unit arithmetic is where the most dangerous mistakes happen, not because any dose is endorsed. No established human dose exists for this compound, and FDA reports no human exposure data for it at all.
One caveat that belongs on the tool rather than buried in a footnote: the arithmetic assumes the vial contains what the label says. Published analysis of products sold as TB500 and TB1000 found content that was not systematically consistent with its descriptions, and this calculator cannot detect that.
Results
- Concentration
- 2.5 mg/mL2500 mcg/mL
- Volume per dose
- 0.1 mL
- U-100 syringe units per dose
- 10 unitsFits the 1 mL barrel (100 unit scale).
- Full doses per vial
- 20 doses
TB-500 has no FDA label and no pharmaceutical-grade formulation, so unlike an approved injectable there are no official mixing constants, no validated diluent, no stated shelf life and no verified vial content to preload. The vial sizes offered as presets below (2, 5 and 10 mg) are the strengths products are commonly labelled with; they are teaching values, not product data and not vendor claims. Published analysis of marketed TB500 and TB1000 products found content that was not systematically consistent with its descriptions, so the calculator's arithmetic assumes a label that nobody has independently verified. The tool accepts any values you choose to document.
Arithmetic only: concentration = vial mg / water mL; units = mL x 100. It does not check any dose against any reference.
Worked example
Worked example (arithmetic, not a recommendation). A vial labelled 5 mg mixed with 2 mL of bacteriostatic water gives 5 / 2 = 2.5 mg/mL, which is 2500 mcg per mL. A documented dose of 250 mcg is 0.25 mg; 0.25 / 2.5 = 0.1 mL. On a U-100 syringe, 0.1 mL x 100 = 10 units. The vial holds 5000 mcg, so floor(5000 / 250) = 20 full draws.
For scale, and to make the substitution concrete: the published human trials of thymosin beta-4, the full 43-amino-acid protein, dosed 42 to 1260 mg intravenously. The largest of those single doses is 126 times the entire contents of a 10 mg TB-500 vial, of a molecule 5.6 times the mass. The two dosing worlds are not describing the same drug, and no validated conversion exists between them.
Step by step
- Enter the peptide content in mg that your vial's label claims. This tool cannot verify it, and published product analysis found labels that did not match contents.
- Enter the volume of bacteriostatic water you add, in mL.
- Read the resulting concentration in mg/mL and in mcg per mL.
- Enter a dose in mcg to see its volume in mL and its mark on a U-100 insulin syringe.
- Read doses per vial as floor(vial content / dose). It assumes the label is accurate.
Arithmetic reference rows (teaching values, not product data)
| Vial (mg) | Water (mL) | mg/mL | mcg per 0.1 mL | 250 mcg dose reads as | 500 mcg dose reads as |
|---|---|---|---|---|---|
| 2 | 1 | 2.00 | 200 | 12.5 units | 25 units |
| 2 | 2 | 1.00 | 100 | 25 units | 50 units |
| 5 | 1 | 5.00 | 500 | 5 units | 10 units |
| 5 | 2 | 2.50 | 250 | 10 units | 20 units |
| 5 | 3 | 1.67 | 167 | 15 units | 30 units |
| 10 | 1 | 10.00 | 1000 | 2.5 units | 5 units |
| 10 | 2 | 5.00 | 500 | 5 units | 10 units |
| 10 | 3 | 3.33 | 333 | 7.5 units | 15 units |
| 10 | 5 | 2.00 | 200 | 12.5 units | 25 units |
Frequently asked questions
Why does the calculator not suggest a dose?
Because none exists to suggest. No human dose-finding study of TB-500 has been published, FDA reports no human exposure data for it, and the one registered study does not disclose its dose levels. The tool documents arithmetic for whatever value you enter; it does not validate that value.
Why are there no dose presets?
Every other input here has presets because vial strengths and diluent volumes are documentable conventions. Dose is different: presetting a number would imply a source exists for it. None does, so the field starts empty.
What do the units on an insulin syringe mean?
U-100 syringes mark 100 units per mL, so 1 unit is 0.01 mL. Units measure volume, not peptide mass; the mass per unit depends entirely on your concentration.
Can I trust the vial strength on the label?
Published analysis of products sold as TB500 and TB1000 found content that was not systematically consistent with its descriptions, and a companion product was mostly sheep extracellular matrix. A certificate of analysis supplied by the seller is a document produced by the seller.
Does peptide stick to the vial or syringe?
It can. TB-500 is one of four model peptides in a published study of adsorption to glassware and plasticware; the finding was that the best consumable depends on the peptide's properties, not on price. A stated concentration is not automatically the delivered concentration.
Tools: educational calculators and references only.